Ethnobotany and phytochemistry of plants used to treat musculoskeletal disorders among Skaw Karen, Thailand.

CONTEXT: Musculoskeletal system disorders (MSD) are prevalent around the world affecting the health of people, especially farmers who work hard in the field. Karen farmers use many medicinal plants to treat MSD. OBJECTIVE: This study collects traditional plant-based remedies used by the Skaw Karen to treat MSD and evaluates their active phytochemical compounds. MATERIALS AND METHODS: The ethnobotanical study was conducted in six Karen villages in Chiang Mai province using semi-structured interviews were of 120 informants. The data were analyzed using ethnobotanical indices including use values (UV), choice value (CV), and informant consensus factor (ICF). Consequently, the 20 most important species, according to the indices, were selected for phytochemical analysis using LC-MS/MS. RESULTS: A total of 3731 use reports were obtained for 139 species used in MSD treatment. The most common ailments treated with those plants were muscular pain. A total of 172 high-potential active compounds for MSD treatment were identified. Most of them were flavonoids, terpenoids, alkaloids, and steroids. The prevalent phytochemical compounds related to treat MSD were 9-hydroxycalabaxanthone, dihydrovaltrate, morroniside, isoacteoside, lithocholic acid, pomiferin, cucurbitacin E, leonuriside A, liriodendrin, and physalin E. Sambucus javanica Reinw. ex Blume (Adoxaceae), Betula alnoides Buch.-Ham. ex D.Don (Betulaceae), Blumea balsamifera (L.) DC. (Asteraceae), Plantago major L. (Plantaginaceae) and Flacourtia jangomas (Lour.) Raeusch. (Salicaceae) all had high ethnobotanical index values and many active compounds. DISCUSSION AND CONCLUSIONS: This study provides valuable information, demonstrating low-cost medicine plants that are locally available. It is a choice of treatment for people living in remote areas.

Clinacanthus nutans genetic diversity and its association with anti-apoptotic, antioxidant, and anti-bacterial activities.

Clinacanthus nutans (Burm. f.) Lindau has been extensively utilized in Thai folk medicine. However, there has been no prior exploration of its genetic diversity or its correlation with biological activity and phytochemical profiles. Herein, a total of 10 samples of C. nutans were collected from different geographic locations in different environments of Thailand, encompassing Northern, Northeastern, and Central regions. The genetic diversity study using sequence-related amplified polymorphism (SRAP) markers showed that all C. nutans samples were closely related, as indicated by UPGMA cluster analysis. When comparing the biological activities of C. nutans extracts, our findings demonstrated that those sourced from Northern Thailand exhibited the most potent activity in reducing lipopolysaccharide-inducing cell death, as accessed by cell viability assay. Furthermore, they showed remarkable antioxidant and antibacterial activities against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. High-performance liquid chromatography (HPLC) analysis of phytochemical profiles revealed consistent chromatography peak patterns across all C. nutans extracts. However, they exhibited varying levels of phenolic contents, as judged by the Folin-Ciocalteu assay, which positively correlated with their observed activities. In conclusion, this study highlights the limited genetic variation within C. nutans population in Thailand. Furthermore, it underscores the association between the biological activity and the total phenolic contents which might be mainly impacted by environmental conditions.

Women's wellness in the mountains: An exploration of medicinal plants among tibeto-burman groups in Thailand.

Ethnopharmacological relevance: This study offers valuable insight into the traditional healing practices of women's healthcare within four Tibeto-Burman groups. By focusing on women's wellness, the research sheds light on the often-neglected yet significant contributions made by women in the field of traditional medicine. Aims of the study: The study seeks to address the gap of studies on the ethnobotany of women healthcare of the Tibeto-Burman groups in Thailand and to analyst the factors that could affect the diversity of ethnobotanical knowledge. Materials and methods: The study compiled data from 15 group-informants residing in 12 different villages. The information gathering process involved a combination of free-listing, semi-structured interviews, group discussion, and the walk-to-the-wood method. To analyze the data, the researchers used various methods such as use-reports, Relative Frequency of Citation (RFC), Relative Importance Index (RI), and Cluster analysis. Results: This study identified 200 plant species used in traditional women's healthcare practices, with leaves being the most frequently used part. Drinking was the most common method for internal use, while bathing and steam were the most emphasized for external use. Blumea balsamifera and Buddleja asiatica had high RI and RFC. Cluster analysis revealed that group-informants were divided by ethnicity and geographical location. Conclusion: This study contributes to the understanding of traditional healthcare practices among Tibeto-Burman groups in Thailand, with a particular focus on women's wellness. The results provide insight into the ethnobotanical knowledge of these communities and highlight the important role played by women in traditional medicine. The study underscores the need for continued research in this field to preserve and utilize this valuable knowledge.

Phytochemical Profiles and Anticancer Effects of Calophyllum inophyllum L. Extract Relating to Reactive Oxygen Species Modulation on Patient-Derived Cells from Breast and Lung Cancers.

Reactive oxygen species (ROS) contribute to cancer growth and metastasis. Using antioxidants to modulate cellular ROS levels is a promisingstrategy for cancer prevention and treatment. Calophyllum inophyllum L., or tamanu, is a medicinal plant renowned for its anti-inflammatory, antioxidant, and anticancer properties in traditional medicine systems. However, the anticancer effects of C. inophyllum extract on cellular ROS remain unexplored. This study represents the first report on such effects and provides the potential mechanisms underlying the anticancer properties of C. inophyllum extract. The branches of C. inophyllum were extracted, and the extract was comprehensively analyzed for phytochemical constituents, antioxidant capacity, total phenolic content, and total flavonoid content. Subsequently, the extract's potential anticancer properties were evaluated using patient-derived cells from breast and lung cancer. The results revealed that the C. inophyllum extract possesses notable antioxidant activity and demonstrated no cytotoxicity within the initial 24 h of treatment. However, after 72 h, it exhibited significant antiproliferative effects. Moreover, the extract exhibited inhibitory properties against migration and invasion at concentrations below the IC50, which corresponded to the expression of related genes. Notably, these effects correlated with the reduction of intracellular ROS levels. Overall, our findings highlight the anticancer potential of C. inophyllum extract, emphasize its ability to modulate cellular ROS levels and target key molecular pathways involved in cancer progression. This study sheds light on the promising therapeutic implications of C. inophyllum extract as a novel agent for cancer treatment, which is safe for normal cells.

Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis.

Background: Cancer is one of the top two leading causes of death worldwide. Ethnobotanical research, it is one of methods, which is able to discover effective anticancer drugs based on "prototype" of indigenous people's historical experiences and practices. The rhizomes of Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz. have been used as botanical drugs to treat cancer by Yi, Bai, Dai, and Naxi ethnic groups in Yunnan, China, where this species is widely cultivated in a large scale in Yunnan. Materials and methods: To identify the substances of anticancer activities based on indigenous medicine knowledge, chromatography was performed to separate saponins from the rhizomes of P. polyphylla var. yunnanensis, followed by spectroscopy to determine the structure of six isolated saponins. The cytotoxicity of five extracts and six pure compounds were evaluated by MTS method. Quantitative determination of total saponins of P. polyphylla var. yunnanensis was analyzed by HPLC. Cell cycle assay, apoptosis assay, and mitochondrial membrane potential were used to evaluate the pro-apoptotic activity in vitro. Results: Five extracts and six pure saponins showed significant inhibitory cytotoxic activities of three human liver cancer cell lines (SMMC-7721, HepG2, and SK-HEP-1) and one non-small-cell lung cancer cell line (A549). The contents of Paris saponins I, II, and VII were 6.96% in the rhizomes of P. polyphylla var. yunnanensis, much higher than Chinese Pharmacopoeia standards (0.6%). Six saponins induced significant apoptosis and cell cycle arrest in three human cancer cell lines (A549, SMMC-7721, and HepG2), which was associated with the loss of mitochondrial membrane potential. Conclusion: The result of this study support that cultivated P. polyphylla var. yunnanensis could be a substitute for wild resource as an anticancer medicine based on indigenous medicine knowledge.

Indian genetic heritage in Southeast Asian populations.

The great ethnolinguistic diversity found today in mainland Southeast Asia (MSEA) reflects multiple migration waves of people in the past. Maritime trading between MSEA and India was established at the latest 300 BCE, and the formation of early states in Southeast Asia during the first millennium CE was strongly influenced by Indian culture, a cultural influence that is still prominent today. Several ancient Indian-influenced states were located in present-day Thailand, and various populations in the country are likely to be descendants of people from those states. To systematically explore Indian genetic heritage in MSEA populations, we generated genome-wide SNP data (using the Affymetrix Human Origins array) for 119 present-day individuals belonging to 10 ethnic groups from Thailand and co-analyzed them with published data using PCA, ADMIXTURE, and methods relying on f-statistics and on autosomal haplotypes. We found low levels of South Asian admixture in various MSEA populations for whom there is evidence of historical connections with the ancient Indian-influenced states but failed to find this genetic component in present-day hunter-gatherer groups and relatively isolated groups from the highlands of Northern Thailand. The results suggest that migration of Indian populations to MSEA may have been responsible for the spread of Indian culture in the region. Our results also support close genetic affinity between Kra-Dai-speaking (also known as Tai-Kadai) and Austronesian-speaking populations, which fits a linguistic hypothesis suggesting cladality of the two language families.

Evaluation on Antidiabetic Properties of Medicinal Plants from Myanmar.

OBJECTIVES: To explore the effective and safe medicines for treating diabetes. METHODS: Hydroalcoholic extracts of 130 medicinal plants belonging to 66 families were evaluated using porcine pancreatic lipase (PPL) inhibition and glucose uptake methods together with a literature review. RESULTS: The extracts of 22 species showed the PPL inhibition activity; 18 extracts of 15 species stimulated glucose uptake in 3T3-L1 adipocytes. Among them, Mansonia gagei J.R. Drumm., Mesua ferrea L., and Centella asiatica (L.) Urb. exhibited both activities. The extracts of Caladium lindenii (André) Madison rhizomes and Azadirachta indica A. Juss. leaves presented the utmost lipase inhibitory activity with IC50 of 6.86 ± 0.25 and 11.46 ± 0.06 µg/mL, respectively. The extracts of Coptis teeta Wall. rhizomes and Croton tiglium L. seeds stimulated the maximum glucose uptake. Ten species are reported to have antidiabetic activity for the first time. Flavonoids and triterpenoids are the dominant antidiabetic compounds in selected medicinal plants from Myanmar. CONCLUSIONS: P. zeylanica, L. cubeba, H. crenulate, M. gagei, C. teeta, and M. ferrea are worthy to advance further study according to their strong antidiabetic activities and limited research on effects in in vivo animal studies, unclear chemical constitutes, and safety.

Ethnochemometric of plants traditionally utilised as local detergents in the forest dependent culture.

The purpose of this study is to access the existing awareness of nearly forgotten Thai detergent plants by the use of chemometrics tool. A Northern Thai forest dependent community was chosen as it played vital role on knowledge retaining of plant utilisations. For initial perception, ethnobotanical survey was conducted to determine usage of plants by the community. Then the utilised plant parts were screened for phytochemicals and their relationships with the defined cleansing terms (viz., shampoo, scrub, detergent, soap, scent and spiritual) were analysed by Principal Component Analysis (PCA). From the results, the most cited plants as known, used and found were Acacia concinna, Clitoria ternetea, Oryza sativa and Citrus hystrix. Biometric analyses advised that knowledge of detergent plant utilisation was well preserved at all age ranges and it was not variable with genders. Cluster analysis described that term 'spiritual' was not narrated with cleansing properties. For phytochemical analysis, plant extracts showed positive variable of bioactive ingredients and the main compounds in the extracts was saponins. These findings confirmed that the knowledge of indigenous plant utilisation was reserved by the forest dependent community and the information is beneficial toward local plant conservation movement.

Autosomal Microsatellite Investigation Reveals Multiple Genetic Components of the Highlanders from Thailand.

The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiatic-speaking (AA) Khmu, Htin and Lawa; the Hmong-Mien-speaking (HM) IuMien and Hmong; and the Sino-Tibetan-speaking (ST) Akha, Karen, Lahu and Lisu. Except the Lawa, the rest of the hill tribes migrated into their present habitats only very recently. The Thai hill tribes were of much interest to research groups focusing on study of cultural and genetic variation because of their unique languages and cultures. So far, there have been several genetic studies of the Thai hill tribes. However, complete forensic microsatellite database of the Thai hill tribes is still lacking. To construct such database, we newly generated 654 genotypes of 15 microsatellites commonly used in forensic investigation that belong to all the nine hill tribes and also non-hill tribe highlanders from northern Thailand. We also combined 329 genotypes from previous studies of northern Thai populations bringing to a total of 983 genotypes, which were then subjected to genetic structure and population relationships analyses. Our overall results indicated homogenous genetic structure within the HM- and Tai-Kadai (TK)-speaking groups, large genetic divergence of the HM-speaking Hmong but not IuMien from the other Thai groups, and genetic heterogeneity within the ST- and AA-speaking groups, reflecting different population interactions and admixtures. In addition to establishing genetic relationships within and among these populations, our finding, which provides a more complete picture of the forensic microsatellite database of the multiple Thai highland dwellers, would not only serve to expand and strengthen forensic investigation in Thailand, but would also benefit its neighboring countries of Laos and Myanmar, from which many of the Thai hill tribes originated and where large populations of these ethnic groups still reside.

Nutrient and Mineral Compositions of Wild Leafy Vegetables of the Karen and Lawa Communities in Thailand.

Wild food plants are commonly used in the traditional diets of indigenous people in many parts of the world, including northern Thailand. The potential contribution of wild food plants to the nutrition of the Karen and Lawa communities remains poorly understood. Wild food plants, with a focus on leafy vegetables, were ranked by the Cultural Food Significance Index (CFSI) based on semi-structured interviews. Twelve wild plant species were highly mentioned and widely consumed. The importance of the wild vegetables was mainly related to taste, availability, and multifunctionality of the species. Their contents of proximate and minerals (P, K, Na, Ca, Mg, Fe, Mn, Zn, and Cu) were analyzed using standard methods. The proximate contents were comparable to most domesticated vegetables. The contents of Mg (104 mg/100 g FW), Fe (11 mg/100 g FW), and Zn (19 mg/100 g FW) in the wild leafy vegetables were high enough to cover the daily recommended dietary allowances of adults (19-50 years), whereas a few species showed Mn contents higher than the tolerable upper intake level (>11 mg/100 g edible part). The wild leafy vegetables, therefore, are good sources of minerals and we recommend their continued usage by indigenous people. Further research on these wild leafy vegetables' contents of antioxidants, vitamins, heavy metals, anti-nutrient factors, and food safety is recommended.

Medicinal Plants for Treating Musculoskeletal Disorders among Karen in Thailand.

Millions of people suffer from Musculoskeletal System Disorders (MSDs), including Karen people who work hard in the fields for their subsistence and have done so for generations. This has forced the Karen to use many medicinal plants to treat MSDs. We gathered data from 15 original references covering 27 Karen communities and we document 461 reports of the use of 175 species for treating MSDs among the Karen people in Thailand. The data were analyzed by calculating use values (UV), relative frequency of citation (RFC) and informant consensus factor (ICF). Many use reports and species were from Leguminosae and Zingiberaceae. Roots and leaves were the most used parts, while the preferred preparation methods were decoction and burning. Oral ingestion was the most common form of administration. The most common ailment was muscle pain. Sambucus javanica and Plantago major were the most important species because they had the highest and second-highest values for both UV and RFC, respectively. This study revealed that the Karen people in Thailand use various medicinal plants to treat MSDs. These are the main resources for the further development of inexpensive treatments of MSDs that would benefit not only the Karen, but all people who suffer from MSD.

Ethnomedicinal Plant Knowledge of the Karen in Thailand.

The Thai Karen, the largest hill-tribe in Thailand, guard substantial ethnomedicinal plant knowledge, as documented in several studies that targeted single villages. Here, we have compiled information from all the reliable and published sources to present a comprehensive overview of the Karen ethnomedicinal plant knowledge. Our dataset covers 31 Karen villages distributed over eight provinces in Thailand. We used the Cultural Importance Index (CI) to determine which species were the most valuable to the Karen and the Informant Consensus Factor (ICF) to evaluate how well distributed the knowledge of ethnomedicinal plants was in various medicinal use categories. In the 31 Karen villages, we found 3188 reports of ethnomedicinal plant uses of 732 species in 150 plant families. Chromolaena odorata, Biancaea sappan, and Tinospora crispa were the most important medicinal plants, with the highest CI values. The Leguminosae, Asteraceae, Zingiberaceae, Euphorbiaceae, Lamiaceae, Acanthaceae, Apocynaceae, and Menispermaceae were the families with the highest CI values in the mentioned order. A high proportion of all the 3188 Karen use reports were used to treat digestive, general and unspecified, musculoskeletal, and skin disorders.

Asian elephant self-medication as a source of ethnoveterinary knowledge among Karen mahouts in northern Thailand.

ETHNOPHARMACOLOGICAL RELEVANCE: Ethnoveterinary medicine is often assumed to be a subset of human medicinal knowledge. Here we investigate the possibility that some ethnoveterinary medicine rather originates from observations of animal self-medication. We document and analyze the ethnoveterinary medicine used by Karen mahouts for elephant care and attempt to determine whether this knowledge originated from humans or elephants. MATERIALS AND METHODS: Elephant camp owners and mahouts in four communities in northern Thailand were interviewed about their knowledge and use of plants for ethnoveterinary elephant care. For each ethnoveterinary plant, data were collected on Karen human medicinal uses and whether elephants independently consume them. Based on overlaps between ethnoveterinary use, human medicinal use and elephant dietary use, plants were classified into three categories: those that originated from Karen human medicine, those that originated from Asian elephant self-medication, and those which were present in both human and elephant knowledge traditions. RESULTS: The use of 34 plants (32 identified at least to genus) and two additional non-plant remedies (salt and human urine) were reported to be used in ethnoveterinary elephant medicine. A total of 44 treatments in 11 use categories were recorded: tonic, wounds, compress, eye problems, indigestion, broken bones, galactagogue, snakebite, fatigue, skin and musth regulation. Of the ethnoveterinary plants, 55% had the same use in human medicine, 43% had different uses and 2% had no use. Elephants consume 84% of the ethnoveterinary plants as part of their natural diet. DISCUSSION: Analysis indicates that 32% of plant uses likely originated from Karen human medicine, 60% of plant uses likely existed independently in both human and elephant knowledge systems, and 8% of plant uses likely originated from elephant self-medicating behavior. The tonic use category shows the strongest evidence of influence from observations of elephant self-medication. The use of tonic medicines appears to be increasing as a way to mitigate the unnaturally limited diet of elephants in tourist camps. CONCLUSION: Ethnoveterinary medicine for elephant care is influenced by both human medicinal knowledge and elephant knowledge of plants for self-medication. The ethnoveterinary knowledge domain appears to be the result of an interactive process linked to convergent evolution or co-evolution between humans and Asian elephants.

Traditional Uses of Leguminosae among the Karen in Thailand.

Leguminosae (legumes) are one of the largest plant families. They are widely used for a variety of purposes by people around the world and include many important cultivated economic food crops. On local scales, legumes are commonly used by various ethnic groups. However, the data are incomplete and scattered, not least in Thailand. We found that species of legumes were important in Karen communities, so we decided to investigate in detail the traditional knowledge of legumes on a local scale among Karen people in northern Thailand. We interviewed six herbalists and eighty-four nonherbalist informants in three Karen villages in Chiang Mai province about their use of legumes, and about the local names for the species, using semistructured interviews. A total of 83 legumes species (in 45 genera) had 4443 use reports. Five of the 83 legume species had not been reported previously as used in Thailand. Most Karen use reports (43%) of legumes were for food, medicine (36%), and materials (8%), but in term of species more legumes (68 species) were used for medicine than for food (53 species). The legume genera with most used species were Crotalaria and Flemingia each with six species. The most important species are Tamarindus indica (CI = 3.38), Senegalia rugata (CI = 2.39), Glycine max (CI = 1.27) respectively.

Partial rpoB Gene Sequencing Identification and Probiotic Potential of Floricoccus penangensis ML061-4 Isolated from Assam Tea (Camellia sinensis var. assamica).

Assam tea or Miang is a local name of Camellia sinensis var. assamica in northern Thailand. By the local wisdom, Assam tea leaves are used as the raw material in tea fermentation to produce "Fermented Miang" consumed by people in northern Thailand and the countries nearby. In this study, twenty-eight bacterial isolates were obtained from Assam tea leaf samples collected from Nan province, Thailand. Bacterial isolates were identified within 6 genera including Bacillus, Floricoccus, Kocuria, Lysinibacillus, Micrococcus and Staphylococcus. Among these, the strain ML061-4 shared 100.0 and 99.4% similarity of 16S rRNA and rpoB gene sequence with F. penangensis JCM 31735T, respectively. This is the first discovery of F. penangensis in Thailand. F. penangensis ML061-4 exhibited probiotic characteristics including lactic acid production (9.19 ± 0.10 mg/ml), antibacterial activities (Escherichia coli ATCC 25922 and E. coli O157:H7 DMST 12743), acid and bile salt tolerance (71.1 and 54.9%, respectively), autoaggregation (97.0%), coaggregation (66.0% with E. coli O157:H7), cell surface hydrophobicity (90.0%), bacterial adhesion (82.9% with Lactobacillus plantarum FM03-1), competitive inhibition (17.8% with E. coli O157:H7) and competitive exclusion (34.9% with E. coli O157:H7). Overall, the data suggested that F. penangensis ML061-4 had a great potential to be a probiotic.

Exotic Plants Used by the Hmong in Thailand.

Exotic species are an integral part of the plants used by many ethnic groups, but they usually receive little attention and have been considered alien to the ethnobotanical data. Here, we analyze the plants used by Thai Hmong refugees that are not native to their current habitats in Thailand. We attempt to understand the sources of this knowledge. Do people maintain the original traditional knowledge related to exotic species when they migrate to a new region, or does new knowledge originate from acculturation? We interviewed 16 specialist Hmong informants in Nan province, Thailand, about their traditional knowledge of 69 exotic species used. Acquisition of this knowledge has a long history; several species are the same as plants used by the Hmong in China and other countries, others are globally useful species which have become part of the pool of species that the Hmong have developed local knowledge about. However, migration also involves the integration of local knowledge from other cultures, and also adapts them to function in urban settings. This includes using closely related exotic taxa that replace some of the species they used in their original homelands. The migrants' traditional knowledge in their new habitats is more complicated and also involves the development of local knowledge that is entirely new.

Medicinal plants in homegardens of four ethnic groups in Thailand.

ETHNOPHARMACOLOGICAL RELEVANCE: Homegardens are important habitats for medicinal plants and traditional knowledge, especially among indigenous groups in remote areas. In homegardens, medicinal plants and traditional knowledge are well conserved through human management and ease of access. AIM OF THE STUDY: To understand the drivers of diversity and the composition of homegardens with focus on medicinal plants, including exotic species, and their uses. We compare the homegardens of four ethnic groups in northern Thailand (Thai Yuan, Lahu, Karen, and Lisu). METHODS: We inventoried all medicinal species in 195 homegardens from four villages belonging to the four ethnic groups. The owners were asked to point out all useful species in their homegardens. For any medicinal species, they were then interviewed about their main and secondary uses, medicinal properties, plant part used, preparation methods, and route of administrations. RESULTS: We recorded 95 medicinal homegarden plant species ranging from 20-59 species per village and ethnic group. Most of the medicinal plants had a primary use as food plants, which demonstrated the close relationship between local food and medicine in the lives of these ethnic groups. Many of the medicinal plants were also used as food additives, ornamentals, or materials, showing that medicinal plants are not an exclusive category in the homegardens. The number of homegarden plants varied almost four-fold from 58 species in one village to 211 in the most species rich village. The number of medicinal homegarden plants varied accordingly from 20 to 59 in the poorest and the richest villages. Five medicinal species were found in all villages, but 70% of the medicinal plants were found in only one village. Infection & infestation, Nutritional disorders, and Digestive system disorders were the three most important medicinal use categories for plants in the homegardens, and all three categories were found in most of the studied homegardens. Of the 95 species with medicinal uses, 26 (27%) were exotic, which shows that local people amplify the spectrum of alternative ways to treat their illnesses. CONCLUSION: Homegardens are an important source of ethnomedicinal species and knowledge. These species are beneficial for treating acute and common ailments; they are easy to access and can be prepared by simple methods, which are suitable for treating disorders, such as infections or any digestive system ailments. Medicinal plants in homegardens have been important in improving health, at least, at the family level.

Chemical diversity and anti-acne inducing bacterial potentials of essential oils from selected Elsholtzia species.

Essential oils from the aerial parts of four Elsholtzia species; Elsholtzia stachyodes, Elsholtzia communis, Elsholtzia griffithii and Elsholtzia beddomei were obtained by steam distillation and their chemical components were analysed by gas chromatography-mass spectrometry (GC-MS). Principle Component Analysis was used to identify the chemical variations in the essential oils from these plants, which could be categorised into two groups according to their main chemical components which are acylfuran derivatives and oxygenated monoterpenes. Additionally, the anti-acne inducing bacterial activity against Staphylococcus aureus and Staphylococcus epidermidis were evaluated. The oil from E. stachyodes was the most efficacious against the growth of S. aureus and S. epidermidis having MIC values of 0.78 and 1.56 µL/mL, respectively, and exhibited five times more effective than erythromycin (standard antibiotic).

Ethnomedicinal plant diversity in Thailand.

ETHNOPHARMACOLOGICAL RELEVANCE: Plants have provided medicine to humans for thousands of years, and in most parts of the world people still use traditional plant-derived medicine. Knowledge related to traditional use provides an important alternative to unavailable or expensive western medicine in many rural communities. At the same time, ethnomedicinal discoveries are valuable for the development of modern medicine. Unfortunately, globalization and urbanization causes the disappearance of much traditional medicinal plant knowledge. AIM OF THE STUDY: To review available ethnobotanical knowledge about medicinal plants in Thailand and to estimate its diversity. METHODS: Information about ethnomedicinal uses of plants in Thailand was extracted from 64 scientific reports, books, and theses produced between 1990 and 2014. Plant identifications in the primary sources were updated to currently accepted names following The Plant List website and the species were assigned to family following the Angiosperm Phylogeny Website. Use Values (UV) were calculated to estimate the importance of medicinal plant species (UVs) and families (UVf). Medicinal use categories, plant parts used, preparations of the medicine, and their applications were noted for each use report. RESULTS: We found 16,789 use reports for 2187 plant species in 206 families. These data came from 19 ethnic groups living in 121 villages throughout Thailand. The health conditions most commonly treated with medicinal plants were in the categories digestive system disorders, infections/infestations, nutritional disorders, muscular-skeletal system disorders, and genitourinary system disorders. Plant families with very high use values were Fabaceae, Asteraceae, Acanthaceae, Lamiaceae, and Zingiberaceae and species with very high use values were Chromolaena odorata (L.) R.M.King & H.Rob., Blumea balsamifera (L.) DC., and Cheilocostus speciosus (J.Koenig) C.D.Specht. Stems and leaves were the most used plant parts, but also other parts of the plants were used in medicinal recipes. The most common way of using the medicinal plants was as a decoction in water. CONCLUSION: We found 2187 plant species that were used in traditional medicine in Thailand. Of these a few hundred had high use values, suggesting that they may produce bioactive compounds with strong physiological effects.

Karen and Lawa medicinal plant use: uniformity or ethnic divergence?

ETHNOPHARMACOLOGICAL RELEVANCE: We here tease apart the ethnopharmacological knowledge of plants in two Thai villages to determine to which degree the uses are particular to individual ethnic groups and to which degree they are part of a generalized and uniform set of widespread medicinal plants used over a large geographic range. We compared Karen and Lawa knowledge of medicinal plants in the Mae Cheam watershed of northern Thailand, where both ethnic groups have settled and share ecological conditions for resource extraction. We were interested in documenting the degree to which these two ethnic groups use the same or different medicinal plant species. The use of the same plant species by the two groups was considered a sign of uniform and cross-cultural local knowledge, whereas the use of different medicinal plants by each group was considered a sign of culturally specific local knowledge that developed within each ethnic group. MATERIALS AND METHODS: We inventoried the plant species in different habitats around one Karen village and one Lawa village using stratified vegetation plots and using semi-structured questionnaires we interviewed 67 key informants regarding their use of plants for medicine. We then calculated the Fidelity level FL (FL values near 100% for a species indicate that almost all use reports refer to the same way of using the species, whereas low FL values indicate that a species is used for many different purposes) and cultural importance index CI (the sum of the proportion of informants that mention each of the use categories for a given species) to estimate the variation in medicinal plant use. We used Jaccard's Index JI (This index relates the number of shared species to the total number of species) to analyze the similarity of medicinal plant use between the two villages. RESULTS: A total of 103 species of medicinal plant species in 87 genera and 41 families were identified and they were used to cure 35 ailments. The FL of the medicinal plant species varied from 10% to 100%, was different for each ailment, and differed between the two ethnic groups. The most important medicinal plant species, those with the highest CI value, were not the same in the two villages. Costus speciosus, which is used to treat urinary infections and wounds in animals, had the highest CI value in the Karen village, whereas Sambucus javanica, which is used to treat wounds, fractures, bloat, and edema in humans, had the highest CI value in the Lawa village. Only 17 medicinal species (16.5%) were shared between the two villages. Methods of preparation and application were significantly different between the two villages, whereas the plant parts used, habit, and route of administration were similar. CONCLUSION: Our study demonstrates that ethnic groups that live in the same geographic area can have significantly different traditional knowledge systems for medicinal plants, at least when it comes to the species used and their preparation and medicinal application. We assume that differences in cultural history and background in the two villages led to differences in medicinal plant use, preparation, and application.